For your convenience Out-Grow LLC has made an all in one manure based mushroom grow bag solution to simplify the gourmet mushroom cultivation process. You will receive 2 All in One Grow Bags with this purchase.This all in one grow bag has different layers of substrates so there is no need to transfer substrates to different bags or trays thus reducing the chance of contamination. The all in one grow bag is horse manure based for all dung loving gourmet mushroom species. Fully hydrated and sterilized with a five micron filter patch and self healing injection port. Simply inoculate the rye berry layer of the substrate with 2-5 cc of your favorite gourmet mushroom spore solution. You must provide your own gourmet mushroom culture as none is provided. This sale is for a bag of sterilized substrate only. After inoculation allow your all in one bag to colonize in room temps for 4-6 weeks until your first flush of mushrooms. Mixing the bag once after 50% colonization is optional and can increase colonization times. These bags hold three pounds (about 4 quarts of substrate).